Affinity extraction of proteins from acidic environments (979.2)

extraction and acetylation of purified trypsin from bovin pancreas and study of some its physico-chemical properties

آنزیم تریپسین در شرایط قلیایی ناپایدار می باشد .و فعالیت پروتئولیتیکی تریپسین منجربه خود هضمی آن در جایگاههای خاصی می گردد. بنابر این آنزیمی با ناپایداری بالا محسوب میگردد. در سالهای اخیر موفق شدند که با ایجاد تغیرات شیمیایی با اضافه کردن فلزات خاص ، کلسیم و یا عمل استیلاسیون منجر به افزایش پایداری آنزیم تریپسین گردند. مطالعات در حال حاضر نشان می دهد که تریپسین استیله شده فعالیت آنزیمی خود را ...

Solvent Extraction of Zinc from Acidic Solution Obtained from Cold Purification Filter Cake of Angouran Mine Concentrate Using D2EHPA

Microwave Assisted Extraction of Olive Oil Pomace by Acidic Hexane

In this study, Microwave-Assisted Solvent Extraction (MASE) was used to recover oil residues from pomace olive using acidic hexane. Results obtained demonstrated that oil extraction yield increased with time, the amount of acetic acid in hexane and power radiation. For both radiation powers used (170 and 510W), the optimal extraction time and most interesting content of acetic acid in hexan...

Affinity Based Nano-Magnetic Particles for Purification of Recombinant Proteins in Form of Inclusion Body

Background: Protein purification is the most complicated issue in the downstream processes of recombinant protein production; therefore, improved selective purification methods are important. Affinity-based protein purification method using His-tag and Ni-NTA resins is one of the most common strategies. MNPs can be used as a beneficial alternative for Ni-NTA resins. However, there is no data on...

Role of Acidic Chromatin Proteins

The multiple classes ofbinding sites for the progesterone-receptor complex in hen oviduct nuclei were found to be of chromatin origin. The highest-affinity, and presumably most physiologically important class, is localized in oviduct chromatin and contains approx. 6000-10000 sites per nucleus. None of these sites is detected in spleen chromatin. Two new techniques were used for assaying rapidly...